Mouse Insulin Like Growth Factor-1 Recombinant
Categories: IGF familyIGF familyRecombinant Mouse Cytokines$70.00 – $1,350.00
Description
Accession
P05017
Source
Optimized DNA sequence encoding mouse Insulin Like Growth Factor-1 mature chain was expressed in Escherichia Coli.
Molecular weight
Native Mouse IGF-1, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8 kDa. Recombinant IGF1 is a monmer protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkeyMBr-5 cells was found to be in the range of.0-40.0 ng/ml.
Protein Sequence
MGKISSLPTQ LFKICLCDFL KIKIHIMSSS HLFYLALCLL TFTSSTTAGP ETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKAA RS IRAQRHTDMP KTQKEVHLKN TSRGSAGNKT YRM
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse IGF-1 was lyophilized from a 0.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
LPLI regulates different osteogenic differentiation markers.
- IGF1.
In vitro differentiation of Nes-GFP+ cells
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LC differentiation For LC lineage differentiation, theNes -GFP+ cells were replated in fresh differentiation-inducing medium containing phenol red-free DMEM/F12, 2% FCS, 10 ng/ml PDGF-BB , 1 ng/ml LH , 1 nM thyroid hormone , 70 ng/ml insulin-like growth factor 1 (IGF1), and ITS supplement , and they were incubated for 7 days, as previously described. - Differentiation was subsequently confirmed by RT-PCR and immunostaining for LC lineage markers (antibodies shown in
