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Human Tumor Necrosis Factor-beta Recombinant

TNF

accession:  P01374
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2800
Domain  : 
Gene  :  LTA
Catalog no. :  RKP01374
Source:
Optimized DNA sequence encoding Human TNF beta mature chain was expressed in Escherichia Coli

 
Molecular weight:
Native human Tumor Necrosis Factor-beta is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 19kDa.
Recombinant TNF-beta is a non disulfide-linked homotrimer protein,each consisting of 172 amino acids and migrates as an approximately 21 kDa protein under reducing conditions.

 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was Measured in a cytotoxicity assay using mouse L929 cells in the presence of the metabolic inhibitor actinomycin, and was determined to be less than 0.1ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MTPPERLFLP RVCGTTLHLL LLGLLLVLLP GAQGLPGVGL TPSAAQTARQ HPKMHLAHST 

        70         80         90        100        110        120 
LKPAAHLIGD PSKQNSLLWR ANTDRAFLQD GFSLSNNSLL VPTSGIYFVY SQVVFSGKAY 

       130        140        150        160        170        180 
SPKATSSPLY LAHEVQLFSS QYPFHVPLLS SQKMVYPGLQ EPWLHSMYHG AAFQLTQGDQ 

       190        200 
LSTHTDGIPH LVLSPSTVFF GAFAL 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
TNF-beta is presented as a 0.2 μm filtered PBS solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

TNF-beta

TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-α, TNF-β is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF- β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells.

Related Publications:
tnfβ+250 polymorphism and hyperdynamic state in cardiac surgery with extracorporeal circulation
Interactive CardioVascular and Thoracic Surgery, Dec 2008; 7: 1071 - 1074.
mycoplasma fermentans and tnf- interact to amplify immune-modulating cytokines in human lung fibroblasts
Am J Physiol Lung Cell Mol Physiol, Oct 2006; 291: L781 - L793.
il-10 and tnf-ß gene polymorphisms have no major influence on lactate levels after cardiac surgery
Eur. J. Cardiothorac. Surg., Jul 2006; 30: 54 - 58.
effect of tnf and tnfr 2 polymorphisms, –308 (g/a) and +489 (g/a) of tnf-alpha gene, +245 (g/a) of TNF-beta gene and m196r of tnfr 2 gene, on infliximab response in rheumatoid arthritis patients
Ann Rheum Dis, Jun 2005; 64: 376.
il-2-induced tumor necrosis factor (tnf)-ß expression: further analysis in the il-2 knockout model, and comparison with tnf-, lymphotoxin-ß, tnfr1 and tnfr2 modulation
Int. Immunol., Feb 2001; 13: 135 - 147.




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