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Human Superoxide Dismutase Recombinant

SOD

accession:  P00441
   Size A     20ug   $ 70
   Size B      100ug  $160
   Size C      1mg  $ 600
Domain  :  SOD
Gene  :  SOD
Catalog no. :  RKP00441
Source:
Optimized DNA sequence encoding Human Superoxide Dismutase mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human Superoxide Dismutase generated by the proteolytic removal of the signal peptide and propeptide,and has a calculated molecular mass of approximately 17 kDa. 

SOD is a disulfide-linked homodimeric protein consisting of two 154 amino acid residue subunits, and migrates as an approximately 31kDa protein under non-reducing and as 16 kDa under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity was measured by Pyrogallic Acid method and was found to be not less than 1x104 IU/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS 

        70         80         90        100        110        120 
AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV 

       130        140        150 
HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
SOD was lyophilized from a 0.2 μm filtered PBS solution pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Superoxide Dismutase

Superoxide Dismutase has been isolated from media conditioned by a murine marrow-derived cell line (PB6) and from the conditioned medium of cultured mouse marrow cells. It antagonizes IL3 dependent proliferation of cells in culture and reversibly inhibits DNA synthesis of erythroid progenitor cells.

Related Publications:
identification of francisella tularensis live vaccine strain cuzn Superoxide Dismutase as critical for resistance to extracellularly generated reactive oxygen species
J. Bacteriol., Oct 2009; 191: 6447 - 6456.
immature copper-zinc Superoxide Dismutase and familial amyotrophic lateral sclerosis
Experimental Biology and Medicine, Oct 2009; 234: 1140 - 1154.
polymorphism of the manganese Superoxide Dismutase gene but not of vascular endothelial growth factor gene is a risk factor for diabetic retinopathy
Br J Ophthalmol, Oct 2009; 93: 1401 - 1406.
mice deficient in both mn Superoxide Dismutase and glutathione peroxidase-1 have increased oxidative damage and a greater incidence of pathology but no reduction in longevity
J Gerontol A Biol Sci Med Sci, Sep 2009; 10.1093/gerona/glp132.
the right to choose: multiple pathways for activating copper,zinc superoxide dismutase
J. Biol. Chem., Sep 2009; 284: 24679 - 24683.




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Antibodies:
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