Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human Stem Cell Factor Recombinant

SCF

accession:  P21583
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 2700
Domain  :  four helix cytokine core
Gene  :  Kitlg
Catalog no. :  RKP21583
Source:
Optimized DNA sequence encoding Human SCF mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18 kDa.

Recombinant SCF is a disulfide-linked monomer protein consisting of 165 amino acid residue subunits, and migrates as an approximately 18 kDa protein under non-reducing
and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MKKTQTWILT CIYLQLLLFN PLVKTEGICR NRVTNNVKDV TKLVANLPKD YMITLKYVPG 

        70         80         90        100        110        120 
MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI IDKLVNIVDD LVECVKENSS 

       130        140        150        160        170        180 
KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA FKDFVVASET SDCVVSSTLS PEKDSRVSVT 

       190        200        210        220        230        240 
KPFMLPPVAA SSLRNDSSSS NRKAKNPPGD SSLHWAAMAL PALFSLIIGF AFGALYWKKR 

       250        260        270 
QPSLTRAVEN IQINEEDNEI SMLQEKEREF QEV 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant SCF was lyophilized from a 0.2 μm filtered solution inPBS pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

SCF

SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR(CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.

Related Publications:
pollen-specific skp1-like proteins are components of functional SCF complexes and essential for lily pollen tube elongation
Plant Cell Physiol., Aug 2009; 50: 1558 - 1572.
SCF induces -globin gene expression by regulating downstream transcription factor coup-tfii
Blood, Jul 2009; 114: 187 - 194.
jfk, a kelch domain-containing f-box protein, links the SCF complex to p53 regulation
PNAS, Jun 2009; 106: 10195 - 10200.
the extracellular signal-regulated kinase–mitogen-activated protein kinase pathway phosphorylates and targets cdc25a for SCFβ-trcp-dependent degradation for cell cycle arrest
Mol. Biol. Cell, Apr 2009; 20: 2186 - 2195.
mmp9 and SCF protect from apoptosis in acute kidney injury
J. Am. Soc. Nephrol., Apr 2009; 20: 787 - 797.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human Stem Cell Factor Recombinant
product_icon_8.jpg Mouse Stem Cell Factor Recombinant
product_icon_8.jpg Human Stem Cell Factor Recombinant (CHO Cell)
product_icon_7.jpg Monoclonal mouse anti SCF
product_icon_8.jpg Human CD117 Recombinant (SCFR)
product_icon_8.jpg Mouse CD117 Recombinant (SCFR)
product_icon_8.jpg Mouse Stem Cell Factor Recombinant
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $