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Human Platelet Derived Growth Factor-BB Recombinant

PDGF

accession:  P01127
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3500
Domain  :  cystine knot growth factor
Gene  : 
Catalog no. :  RKP01127
Source:
Optimized DNA sequence encoding Human PDGF-BB mature chain was expressed in Saccharomyces cerevisiae.

 
Molecular weight:

Human PDGF-BB, generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 16 kDa . 

Recombinant PDGF-BB is a disulfide-linked homodimeric protein consisting of two 109 amino acid residue subunits. Recombinant  PDGF-BB migrates due to glycosylation as an approximately 32 kDa protein under non-reducing conditions and as 16kDa under reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC.

 
Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of Balb/c 3T3 cells.The expected ED50 for this effect is 1.0-3.0 ng/ml, corresponding to 1x106 IU/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MNRCWALFLS LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHG DPGEEDGAEL 

        70         80         90        100        110        120 
DLNMTRSHSG GELESLARGR RSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV 

       130        140        150        160        170        180 
WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC 

       190        200        210        220        230        240 
ETVAAARPVT RSPGGSQEQR AKTPQTRVTI RTVRVRRPPK GKHRKFKHTH DKTALKETLG 


A 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Platelet Dreived Growth Factor-BB  was lyophilized from a 0.2 μm filtered 0.02M acetic acid solution pH 6.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

PDGF-BB

PDGF is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets. Platelets synthesize a mixture of the three possible isoforms (BB,AB,AA)while fibroblasts stimulated with EGF synthesize AA homodimers. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells, and endothelial cells.Two related receptors, are PDGFRalpha (CD140a) or PDGFRbeta ( CD140b).In contrast to many other cytokines PDGF is not released into the circulation. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix which facilitates local concentration of the factor. The factor functions as a local autocrine and paracrine growth factor. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF regulates the synthesis of its own receptor and also influences the expression of membrane receptors for IL1, EGF, 5-Hydroxytryptamine, LDL, transferrin, and muscarinergic receptor.

Related Publications:
paradoxical effects of PDGF-BB overexpression in endothelial cells on engineered blood vessels in vivo
Am. J. Pathol., Jul 2009; 175: 294 - 302.
sp1-dependent activation of klf4 is required for PDGF-BB-induced phenotypic modulation of smooth muscle
Am J Physiol Heart Circ Physiol, Apr 2009; 296: H1027 - H1037.
mycophenolic acid inhibits the autocrine pdgf-b synthesis and PDGF-BB-induced mrna expression of egr-1 in rat mesangial cells
Nephrol. Dial. Transplant., Jan 2009; 24: 52 - 61.
recombinant PDGF-BB enhances platelet recovery in mice with radiation induced thromobocytopenia.
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 1232.
platelet-derived growth factor (pdgf)-bb mediates survival of leukemic large granular lymphocyte via an autocrine regulatory pathway
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 3803.




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