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Human Neurotrophin-3 Recombinant

NT-3

accession:  P20783
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  NGF
Gene  :  NTF3
Catalog no. :  RKP20783
Source:
Optimized DNA sequence encoding Human Neurotrophin-3 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human Neurotrophin-3 is generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 14 kDa.
 

Recombinant NT-3 is a disulfide-linked homodimer protein consisting of two 120 amino acid residue subunits, and migrates as an approximately 27 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 10-50 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MSILFYVIFL AYLRGIQGNN MDQRSLPEDS LNSLIIKLIQ ADILKNKLSK QMVDVKENYQ 

        70         80         90        100        110        120 
STLPKAEAPR EPERGGPAKS AFQPVIAMDT ELLRQQRRYN SPRVLLSDST PLEPPPLYLM 

       130        140        150        160        170        180 
EDYVGSPVVA NRTSRRKRYA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK 

       190        200        210        220        230        240 
TGNSPVKQYF YETRCKEARP VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI 

       250 
RIDTSCVCAL SRKIGRT 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant NT-3 was lyophilized from a 0.2 μm filtered  PBS solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

Neurotrophin-3

NT-3 is found in neurons of the central nervous system. NT-3 is expressed also in muscles and its expression is down-regulated in denervated muscles. Many human gliomas express and secrete NT-3 into the conditioned medium.ome protein domains of NT-3 are identical with those of NGF and BDNF.NT-3 selectively supports the survival of neuronal cell populations. NT-3 has been shown recently to prevent death of cultured embryonic rat spinal motor neurons at picomolar concentrations. NT-3 has been shown to enhance sprouting of corticospinal tract during development and after adult spinal cord lesion. The activities of NT-3 and BDNF are additive in some systems.The biological activities of NT- 3 are mediated by a receptor belonging to the trk family of receptors with intrinsic tyrosine-specific protein kinase activity. NT-3 only binds weakly to the trk receptor which is a high- affinity receptor for NGF.

Related Publications:
Neurotrophin-3 is involved in the formation of apical dendritic bundles in cortical layer 2 of the rat
Cereb Cortex, Jul 2009; 10.1093/cercor/bhp093.
nerve growth factor and Neurotrophin-3 mediate survival of pulmonary plasma cells during the allergic airway inflammation
J. Immunol., Apr 2009; 182: 4705 - 4712.
functionally reduced sensorimotor connections form with normal specificity despite abnormal muscle spindle development: the role of spindle-derived neurotrophin 3
J. Neurosci., Apr 2009; 29: 4719 - 4735.
complementary actions of bdnf and Neurotrophin-3 on the firing patterns and synaptic composition of motoneurons
J. Neurosci., Jan 2009; 29: 575 - 587.
neurotrophin 3 induces structural and functional modification of synapses through distinct molecular mechanisms
J. Cell Biol., Dec 2006; 175: 1029 - 1042.




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