Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human NAP-2 (CXCL7) Recombinant

NAP-2

accession:  P02775
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 2700
Domain  : 
Gene  : 
Catalog no. :  RKP02775
Source:
Optimized DNA sequence encoding Human NAP-2 (CXCL7) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human NAP2, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 8 kDa.

Recombinant NAP-2 is a disulfide-linked monomeric protein consisting of 71 amino acid residue subunits and migrates as an approximately 8 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MSLRLDTTPS CNSARPLHAL QVLLLLSLLL TALASSTKGQ TKRNLAKGKE ESLDSDLYAE 

        70         80         90        100        110        120 
LRCMCIKTTS GIHPKNIQSL EVIGKGTHCN QVEVIATLKD GRKICLDPDA PRIKKIVQKK 

       130 
LAGDESAD 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant NAP2 was lyophilized from a 0.2 μm filtered 20mM PB,100mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

NAP-2

The chemokine neutrophil- activating peptide 2 (NAP-2) is a 70-amino acid residue polypeptide that is formed from platelet-derived precursors by proteolytic processing.Mature NAP-2 stimulates various effector functions of polymorphonuclear neutrophil granulocytes (PMN) including directed chemotactic migration, exocytosis of lysosomal enzymes and secondary granule contents, and up-regulation of adhesion receptors.NAP-2 has been assigned to a subfamily now termed alpha-chemokines. The alpha-chemokines contain four cysteine residues at highly conserved positions, which enclose the core region of the molecules. The first two cysteines are separated by a single amino acid, forming a motif (CXC) that distinguishes the alpha-chemokine from the beta-chemokine subfamily, where these cysteines are in directly adjacent position.

Related Publications:
relationship between neutrophil-binding affinity and suitability for infection imaging: comparison of 99mtc-labeled NAP-2 (cxcl-7) and 3 c-terminally truncated isoforms
J. Nucl. Med., Jul 2004; 45: 1217 - 1223.
thrombopoietin-induced cxc chemokines, NAP-2 and pf4, suppress polyploidization and proplatelet formation during megakaryocyte maturation
Genes Cells, Jan 2003; 8: 9 - 15.
chemotactic activity of human blood leukocytes in plasma treated with edta: chemoattraction of neutrophils about monocytes is mediated by the generation of NAP-2
J. Leukoc. Biol., Jul 2002; 72: 175.
limited and defined truncation at the c terminus enhances receptor binding and degranulation activity of the neutrophil-activating peptide 2 (NAP-2)
J. Biol. Chem., Mar 1995; 270: 6338.
diverging signal transduction pathways activated by interleukin-8 and related chemokines in human neutrophils: interleukin-8, but not NAP-2 or gro alpha, stimulates phospholipase d activity
Blood, Jan 1995; 85: 522 - 531.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human NAP-2 (CXCL7) Recombinant
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $