Human MIP-1 beta (CCL4) Recombinant, Biotinylated
Categories: Biotinylated proteinsCCL chemokinesRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
P13236
Source
DNA sequence encoding Human MIP-1B mature chain was expressed in Escherichia Coli.
Molecular weight
Native human MIP-1 beta is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately8 kDa. Recombinant MIP-1 beta is a monomer protein consisting of 69 amino acid residue subunits,and migrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Applications
Biotinylated MIP-1B was designed to be used in single step detection in ELISA ,WB.
Protein Sequence
MKLCVTVLSL LMLVAAFCSP ALSAPMGSDP PTACCFSYTA RKLPRNFVVD YYETSSLCSQ PAVVFQTKRS KQVCADPSES WVQEYVYDLE LN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant MIP-1 beta was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P51677
Interactor
P32246
Interactor
P32302
Interactor
P51681
Biological Process
Biological Process
Molecular function
