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Human MCP-1(CCL2) Recombinant

MCP-1

accession:  P13500
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  MCP1
Catalog no. :  RKP13500
Source:
Optimized DNA sequence encoding Human MCP-1(CCL2) mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Human MCP-1, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 9 kDa. Recombinant MCP-1 is a disulfide-linked monomeric protein consisting of 77 amino acid residue subunits and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKVSAALLCL LLIAATFIPQ GLAQPDAINA PVTCCYNFTN RKISVQRLAS YRRITSSKCP 

        70         80         90        100 
KEAVIFKTIV AKEICADPKQ KWVQDSMDHL DKQTQTPKT 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
MCP-1 was  lyophilized from PBS pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:

This cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

MCP-1

MCP-1 belongs to the family of chemotactic cytokines.MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, osteoblastic cells. MCP-1 has been shown to exhibit biological activities other than Chemotaxis. It can induce the proliferation and activation of killer cells known as CHAK. Elevated levels of MCP-1 are observed in atherosclerotic plaques that are rich in macrophages. The factor activates the tumoricidal activity of monocytes and macrophages in vivo. It regulates the expression of cell surface antigens (CD11c, CD11b) and the expression of cytokines (IL1, IL6). MCP-1 is a potent activator of human basophils, inducing degranulation and the release of histamines. In basophils activated by IL3, IL5 or GM-CSF MCP-1 enhances the synthesis of leukotriene C4.

Related Publications:
MCP-1 (monocyte chemotactic protein-1)-induced protein, a recently identified zinc finger protein, induces adipogenesis in 3t3-l1 pre-adipocytes without peroxisome proliferator-activated receptor
J. Biol. Chem., Oct 2009; 284: 27620 - 27628.
role of MCP-1 in tumor necrosis factor--induced endothelial dysfunction in type 2 diabetic mice
Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1208 - H1216.
monocyte chemoattractant protein-1 (MCP-1), not mcp-3, is the primary chemokine required for monocyte recruitment in mouse peritonitis induced with thioglycollate or zymosan a
J. Immunol., Sep 2009; 183: 3463 - 3471.
ccl2/MCP-1 controls parasite burden, cell infiltration, and mononuclear activation during acute trypanosoma cruzi infection
J. Leukoc. Biol., Jul 2009; 10.1189/jlb.0309187.
MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis
Am. J. Respir. Cell Mol. Biol., Jul 2009; 10.1165/rcmb.2008-0423OC.




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Antibodies:
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