Human Fibroblast Growth Factor 10 Recombinant Biotinylated
Categories: Biotinylated proteinsFGF familyFGF family$480.00 – $4,800.00
Description
Accession
O15520
Source
DNA sequence encoding Human Fibroblast Growth Factor-10 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human FGF10 is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 19 kDa. Recombinant Fibroblast Growth Factor is a monomer protein consisting of 170 amino acid residue subunits,and migrates as an approximately 19 kDa protein reducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of BaF3 cells expressing FGF receptors, and was found to be <0.5ng/ml., corresponding to a specific activity of 2.0 x10^7 Units/mg.
Protein Sequence
QAL GQDMVSPEAT NSSSSSFSSP SSAGRHVRSY NHLQGDVRWR KLFSFTKYFL KIEKNGKVSG TKKENCPYSI LEITSVEIGV VAVKAINSNY YLAMNKKGKL YGSKEFNNDC KLKERIEENG YNTYASFNWQ HNGRQMYVAL NGKGAPRRGQ KTRRKNTSAH FLPMVVHS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
RecombinantFGF10 was lyophilized from.2 μm filtered PBS solution, pH7.0 .
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Molecular function
