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Human Interleukin-11 Recombinant

IL-11

accession:  P20809
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  four helix cytokine core
Gene  :  IL11
Catalog no. :  RKP20809
Source:
Optimized DNA sequence encoding Human Interleukin-11 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human IL-11, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa.

Recombinant IL-11 is a disulfide-linked monomer protein consisting of  180 amino acid residue subunits, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of murine T11 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MNCVCRLVLV VLSLWPDTAV APGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL 

        70         80         90        100        110        120 
RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK 

       130        140        150        160        170        180 
TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG 

       190        200 
LHLTLDWAVR GLLLLKTRL 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interleukin-11 was lyophilized from a 0.2 μm filtered PB solution in 2.5% glycine,  pH 4.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-11

IL11 is secreted by bone marrow stromal cells (fibroblasts)and is produced also by a number of mesenchymal cells. The IL11 receptor (alpha chain) utilizes gp130 as its signal transducer, which is also a component of other cytokine receptors. The alpha chain of the IL-11 receptor has been identified independently as Etl2. IL11 promotes primary and secondary immune responses in vitro and in vivo and modulates antigen-specific antibody reactions. IL11 promotes the proliferation of IL6 dependent plasmacytoma cell lines in the presence of neutralizing IL6 antibodies. IL11 also stimulates the T-cell dependent development of IgG-secreting B-cells in spleen cell cultures. IL11 inhibits the differentiation of pre- adipocytes. It induces the synthesis of some acute phase proteins by hepatocytes.

Related Publications:
IL-11 regulates autoimmune demyelination
J. Immunol., Oct 2009; 183: 4229 - 4240.
il-17f-induced IL-11 release in bronchial epithelial cells via msk1-creb pathway
Am J Physiol Lung Cell Mol Physiol, May 2009; 296: L804 - L810.
endogenous IL-11 signaling is essential in th2- and il-13–induced inflammation and mucus production
Am. J. Respir. Cell Mol. Biol., Dec 2008; 39: 739 - 746.
decreased mapk- and pge2-dependent IL-11 production in gi2–/– colonic myofibroblasts
Am J Physiol Gastrointest Liver Physiol, Jun 2007; 292: G1511 - G1519.
interferon- downregulates il-1 and tnf- induced IL-11 secretion by human retinal pigment epithelial cells
Invest. Ophthalmol. Vis. Sci., May 2007; 48: 2524.




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