Human IGFBP-3 Recombinant
Categories: IGF familyIGF familyRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
P17936
Source
Optimized DNA sequence encoding HumanIGFBP-3 mature chain fused to an N-terminal His tag was expressed in CHO Cells.
Molecular weight
Native human IGFBP-3, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 28 kDa. RecombinantIGFBP-3 is a monomer protein consisting of 269 amino acid residue subunits, migrates as an approximately28 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the ability toinhibit IGF-II induced proliferation of MCF-7 and was foundto be≤ 0.2 μg/ml in the presence of 15 ng/ml human IGF-II.
Protein Sequence
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant IGFBP3was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
