Human Interferon-beta 1a Recombinant
Categories: Interferon-IL10 familyRecombinant Human Cytokines$70.00 – $3,500.00
Description
Accession
P01574
Source
Optimized DNA sequence encoding HumanInterferon beta 1a mature chain was expressed in Chinese Hamster Ovary Cells.
Molecular weight
Recombinant human IFN-beta 1a, generated by the proteolytic removal of the signal peptide and propeptide, and has a calculated molecular mass of approximately 19 kDa. Recombinant Interferon beta is a monomeric protein consisting of 166 amino acid residue subunits, and migrates due to glycosylation as an approximately 25kDa protein under reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Theactivity was determined by theviral resistance assay of Human WISH cells was found to be in the range ofx108 IU/mg.
Protein Sequence
MTNKCLLQIA LLLCFSTTAL SMSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR LTGYLRN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant IFN-bawas lyophilized from a 0.2 μm filtered sodium acetate solution pH.8.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P17181
Interactor
Biological Process
Molecular function
Methods
Cytokines and Drugs
- Recombinant human cytokines, IFN-beta (IFN-β), IL-28A, and IL-29, were from .
- The HCV NS5B polymerase inhibitor 2′ C-methyl adenosine (2′CMA) was the gift of D. Olsen and S. Carroll .
