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Human Interferon-gamma Recombinant

IFN-gamma

accession:  P01579
   Size A     20ug   $ 70
   Size B      100ug  $160
   Size C      1mg  $ 1000
Domain  :  4_helix_cytokine_core
Gene  :  IFNG
Catalog no. :  RKP01579
Source:
Optimized DNA sequence encoding Human Interferon gamma mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native Interferon gamma is generated by the proteolytic removal of the signal peptide , the molecule has a calculated molecular mass of approximately 17 kDa.

Recombinant Interferon gamma is a disulfide-linked monomer protein consisting of 144 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by  a viral resistance assay of mouse L929 cells and was found to be ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MKYTSYILAF QLCIVLGSLG CYCQDPYVKE AENLKKYFNA GHSDVADNGT LFLGILKNWK 

        70         80         90        100        110        120 
EESDRKIMQS QIVSFYFKLF KNFKDDQSIQ KSVETIKEDM NVKFFNSNKK KRDDFEKLTN 

       130        140        150        160 
YSVTDLNVQR KAIHELIQVM AELSPAAKTG KRKRSQMLFR GRRASQ 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interferon gamma was lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.2.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IFN-g

IFN-gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFN gamma receptors are expressed on all types of human cells with the exception of mature erythrocytes.IFN-gamma- receptor complexes are rapidly internalized by endocytosis. IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. Interferon-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types. The growth inhibitory activities of IFN-gamma are more pronounced than those of the other interferons. However, the main biological activity of IFN-gamma appears to be immunomodulatory in contrast to the other interferons that are mainly antiviral. n T-helper cells IL2 induces the synthesis of IFN-gamma and other cytokines. IFN- gamma acts synergistically with IL1 and IL2 and appears to be required for the expression of IL2 receptors on the cell surface of T- lymphocytes. Blocking of the IL2 receptor by specific antibodies also inhibits the synthesis of IFN-gamma.

Related Publications:
diminished frequency of nk cells and cd4+IFN-g+ t cells in patients with mycotic corneal ulcers
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1945.
IFN-g-stimulated mesenchymal stromal cells acquire the ability to cross-present exogenous soluble antigens and lead to an effective immune response.
Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 4898.
regulation of tlr expression and activation in human mesenchymal stromal cells by ifn-a, IFN-g and tnf-a.
Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 1790.
positive association of tnf-857 and ifn g 874 gene polymorphisms with chronic fatigue syndrome
Ann Rheum Dis, Jun 2005; 64: 344.
measurement of functional cytomegalovirus (cmv)-specific t cells by interferon-gamma-enzyme-linked immunospot (IFN-g elispot) assay specific for a single immunodominant epitope enables the sensitive evaluation of immune recovery for cmv in patients after allogeneic stem cell transplantation (hsct) with a reduced-intensity regimen (rist).
Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 3157.




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