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Human Granulocyte-Colony Stimulating Factor Recombinant

G-CSF

accession:  P09919
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  four helix cytokine core
Gene  :  CSF3
Catalog no. :  RKP09919
Source:
Optimized DNA sequence encoding Human G-CSF mature chain was expressed in Escherichia Coli

 
Molecular weight:

Native human G-CSF is generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa.

Recombinant GCSF is a disulfide-linked monomer protein consisting of 175 amino acid residue subunits, and migrates as an approximately 19 kDa protein under non-reducing
and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent proliferation of murine M-NFS-60 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 108units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA 

        70         80         90        100        110        120 
   LQEKLCA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG 

       130        140        150        160        170        180 
LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR 

       190        200 
AGGVLVASHL QSFLEVSYRV LRHLAQP 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant G-CSF was lyophilized from a 0.2 μm filtered PBS solution pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

G-CSF

G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is produced also by stromal cells, fibroblasts, and endothelial cells. Epithelial carcinomas, acute myeloid leukemia cells and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM-CSF.Comparison of the primary sequence of G-CSF with those of the two other colony stimulating factors, GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF show a sequence homology of approximately 70 % at the DNA level and of 72 % at the protein level. The G-CSF receptor ,CD114, is expressed on all cells of the neutrophils and granulocytes lineage. It is expressed also in placenta cells, endothelial cells and various carcinoma cell lines.Human G-CSF is active in murine cells and vice versa. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner. G-CSF synergises with some other cytokines, including GM-CSF and IL4. GM-CSF and G-CSF are required, for example, to develop neutrophilic colonies in vitro. The concerted action of G-CSF and Epo is required to support the growth of mixed colonies of the early erythroid progenitors. A combination of IL4 with G-CSF has been shown to lead to synergistic suppression of the growth of some human leukemic cell lines.

Related Publications:
plerixafor (amd3100) and granulocyte colony-stimulating factor (G-CSF) mobilize different cd34+ cell populations based on global gene and microrna expression signatures
Blood, Sep 2009; 114: 2530 - 2541.
the role of granulocyte-colony stimulating factor (G-CSF) in the healthy brain: a characterization of G-CSF-deficient mice
J. Neurosci., Sep 2009; 29: 11572 - 11581.
nfi-a directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls β-globin and G-CSF receptor expression
Blood, Aug 2009; 114: 1753 - 1763.
silencing of microrna-155 in mice during acute inflammatory response leads to derepression of c/ebp beta and down-regulation of G-CSF
Nucleic Acids Res., Jul 2009; 10.1093/nar/gkp577.
preoperative weekly cisplatin–epirubicin–paclitaxel with G-CSF support in triple-negative large operable breast cancer
Ann. Onc., Jul 2009; 20: 1185 - 1192.




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