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Human Bone Morphogenetic protein-2 Recombinant

BMP-2

accession:  P12643
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  Transforming growh factor beta
Gene  :  BMP2
Catalog no. :  RKP12643
Source:
Optimized DNA sequence encoding Human Bone Morphogenetic Protein-2 mature chain was expressed in Escherichia Coli.
 
Molecular weight:

Native human Bone Morphogenetic Protein-2 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately  13 kDa.

Recombinant BMP-2 is a disulfide-linked homodimeric protein consisting of two 114 amino acid residue subunits. BMP2 migrates as an approximately 25 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by its ability to induce alkaline phosphatase production by C2C12 myogenic cells cells was found to be less than  50 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MVAGTRCLLA LLLPQVLLGG AAGLVPELGR RKFAAASSGR PSSQPSDEVL SEFELRLLSM 

        70         80         90        100        110        120 
FGLKQRPTPS RDAVVPPYML DLYRRHSGQP GSPAPDHRLE RAASRANTVR SFHHEESLEE 

       130        140        150        160        170        180 
LPETSGKTTR RFFFNLSSIP TEEFITSAEL QVFREQMQDA LGNNSSFHHR INIYEIIKPA 

       190        200        210        220        230        240 
TANSKFPVTR LLDTRLVNQN ASRWESFDVT PAVMRWTAQG HANHGFVVEV AHLEEKQGVS 

       250        260        270        280        290        300 
KRHVRISRSL HQDEHSWSQI RPLLVTFGHD GKGHPLHKRE KRQAKHKQRK RLKSSCKRHP 

       310        320        330        340        350        360 
LYVDFSDVGW NDWIVAPPGY HAFYCHGECP FPLADHLNST NHAIVQTLVN SVNSKIPKAC 

       370        380        390 
CVPTELSAIS MLYLDENEKV VLKNYQDMVV EGCGCR 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human Bone Morphogenetic Protein-2 was lyophilized from a 0.2 μm filtered solution in 20mM AcOH pH 6.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

bmp-2

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP-2 wild type binds to its cellular receptors via two distinct binding epitopes. The large epitope 1 is responsible for the high- affinity binding to the BMPR-IA receptor, the smaller epitope 2 provides the low-affinity binding to the receptor BMPR-II.

Related Publications:
recombinant human bmp-2 enhances local bone formation in the lumbar spine of osteoporotic sheep
J Bone Joint Surg Br Proceedings, Sep 2009; 91-B: 436.
remodeling of chromatin structure within the promoter is important for bmp-2-induced fgfr3 expression
Nucleic Acids Res., Jul 2009; 37: 3897 - 3911.
bmp-2 is a cost-effective therapy in grade iii open tibia fractures – a health-economic assessment of the use bmp-2 in open tibia fractures for european health care systems
J Bone Joint Surg Br Proceedings, Mar 2009; 91-B: 155.
activation of amp kinase and inhibition of rho kinase induce the mineralization of osteoblastic mc3t3-e1 cells through endothelial nos and bmp-2 expression
Am J Physiol Endocrinol Metab, Jan 2009; 296: E139 - E146.
irbesartan attenuates ang ii-induced bmp-2 expression in human umbilical vein endothelial cells
Vascular Medicine, Aug 2008; 13: 239 - 245.




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Antibodies:
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