Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human Brain-Derived Neurotrophic Factor Recombinant

BDNF

accession:  P23560
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  NGF
Gene  :  BDNF
Catalog no. :  RKP23560
Source:
Optimized DNA sequence encoding Human Brain Derived Neurotrophic Factor mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human native BDNF is generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 14 kDa.

Recombinant BDNF is a disulfide-linked homodimeric protein consisting of two 119 amino acid residue subunits.   migrates as an approximately 27 kDa protein under non-reducing conditions and as a  13-14 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:

The ED(50) was determined by the dose-dependent proliferation of rat C6 cells, and was found to be in the range of 0.5 ug/ml.


 
Protein Sequence:
        10         20         30         40         50         60 
MTILFLTMVI SYFGCMKAAP MKEANIRGQG GLAYPGVRTH GTLESVNGPK AGSRGLTSLA 

        70         80         90        100        110        120 
DTFEHVIEEL LDEDQKVRPN EENNKDADLY TSRVMLSSQV PLEPPLLFLL EEYKNYLDAA 

       130        140        150        160        170        180 
NMSMRVRRHS DPARRGELSV CDSISEWVTA ADKKTAVDMS GGTVTVLEKV PVSKGQLKQY 

       190        200        210        220        230        240 
FYETKCNPMG YTKEGCRGID KRHWNSQCRT TQSYVRALTM DSKKRIGWRF IRIDTSCVCT 


LTIKRGR 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Brain Derived Neurotrophic Factor  was lyophilized from a 0.2 μm filtered citric acid, pH 5.7.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

BDNF

BDNF is found in neurons of the central nervous system. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. The biological activity of BDNF is mediated by a receptor that belongs to the trk family of receptors encoding a tyrosine-specific protein kinase. BDNF only binds weakly to the gp140trk receptor (to which NGF binds with high affinity), and it binds to the NGF receptor known as LNGFR.BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. The factor supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neurons in vitro.BDNF does not appear to act on sympathetic ganglia. In specific neurons of the central nervous system located in the hippocampus and the cortex the synthesis of BDNF is influenced by neuronal activity either positively (glutamate transmitter system) or negatively (GABA transmitter system).

Related Publications:
dendritic trafficking of BDNF mrna is mediated by translin and blocked by the g196a (val66met) mutation
PNAS, Sep 2009; 106: 16481 - 16486.
gabab receptor activation triggers BDNF release and promotes the maturation of gabaergic synapses
J. Neurosci., Sep 2009; 29: 11650 - 11661.
role of exercise-induced BDNF production in the regulation of energy homeostasis
Exp Physiol, Sep 2009; 10.1113/expphysiol.2009.048561.
BDNF val66met polymorphism influences motor system function in the human brain
Cereb Cortex, Sep 2009; 10.1093/cercor/bhp189.
combined application of BDNF to the eye and brain enhances ganglion cell survival and function in the cat following optic nerve injury.
Invest. Ophthalmol. Vis. Sci., Aug 2009; 10.1167/iovs.09-3740.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human Brain-Derived Neurotrophic Factor Recombinant
product_icon_7.jpg Rabbit anti BDNF Polyclonal
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $