Description
Accession
P06725
Source
DNA sequence encoding immunodominant fragment of Cytomegalovirus 65kDa tegument phosphoprotein was expressed in Escherichia Coli.
Molecular weight
Recombinant CMV pp65(UL83) encodes the immunodominant sequences of the Cytomegalovirus matrix phosphoprotein. This molecule has a calculated mass of approximately 22kDa. Recombinant CMV pp65 contains an N terminal GST purification tag and migrates as an approximately 52 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE.
Applications
CMV pp65 antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Presentation
Lyophilized from 25mM Tris-Hcl pH 7.2, 1mM EDTA and 50% glycerol.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
