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Monoclonal mouse anti human TNF-alpha


accession:  P01375
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  SB766
Isotype  :  IgG1
Catalog no. :  RMA01375
  • ELISA:This antibody can be used at 4 μg/mL with the appropriate secondary reagents to detect TNF alpha. The detection limit for recombinant human TNF alpha is approximately 0.1 ng/well.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human TNFa. 
Species reactivity:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.


This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. 



Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF- alpha while CD8(+) cells secrete little or no TNF- alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non- glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF- alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF- alpha forms dimers and trimers.

Related Publications:
tak1-mediated serine/threonine phosphorylation of epidermal growth factor receptor via p38/extracellular signal-regulated kinase: nf-b-independent survival pathways in tumor necrosis factor alpha signaling
Mol. Cell. Biol., Oct 2009; 29: 5529 - 5539.
tumor necrosis factor--mediated regulation of the inositol 1,4,5-trisphosphate receptor promoter
J. Biol. Chem., Oct 2009; 284: 27557 - 27566.
trovafloxacin enhances TNF-induced inflammatory stress and cell death signaling and reduces TNF clearance in a murine model of idiosyncratic hepatotoxicity
Toxicol. Sci., Oct 2009; 111: 288 - 301.
TNF- inhibition attenuates adverse myocardial remodeling in a rat model of volume overload
Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1462 - H1468.
TNF inhibits apoptotic cell clearance in the lung, exacerbating acute inflammation
Am J Physiol Lung Cell Mol Physiol, Oct 2009; 297: L586 - L595.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
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