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Human PD-L1 (B7-H1) Recombinant, Biotinylated

PD-L1

accession:  Q9NZQ7
   Size A     10ug   $ 340
   Size B      25ug  $590
   Size C      100ug  $ 1950
Domain  : 
Gene  :  PDCD1
Catalog no. :  RBQ9NZQ7
Source:
Optimized DNA sequence  encoding  extracellular domain of Human Programmed Death Ligand 1 ,PD-L1, (PHE19 - ARG238) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human PD-L1 was further labeled by Biotin for detection by Avidin conjugated secondary probes.

 
Molecular weight:
Recombinant human PD-L1 (B7-H1) is a protein consisting of 225 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.

 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Applications:
ELISA,WB
Biotinylated recombinant PD-L1 was designed to be used in single step detection in ELISA ,WB
 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human PD-L1 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.

              
Storage:
Recombinant  B7-H1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

PD-L1

The PD-L1, also called B7 homologue 1, is a B7 family protein. It mediates immune tolerance by binding to the PD-1 on activated T cells. PD-1 has two ligands, PD-L1 (B7-H1 or CD274) and PD-L2 (B7-DC or CD273). PD-L2 expression is restricted to activated dendritic cells (DC) and macrophages, whereas PD-L1 is constitutively expressed on a wide variety of cells. Furthermore, PD-L1 expression on cells including APC is up- regulated after stimulation with IFN-γ and toll-like receptor ligands .

Related Publications:
negative regulation by PD-L1 during drug-specific priming of il-22–secreting t cells and the influence of pd-1 on effector t cell function
J. Immunol., Feb 2014; 10.4049/jimmunol.1302720.
PD-L1 expression in primary clear cell renal cell carcinomas (ccrccs) and their metastases.
ASCO Meeting Abstracts, Feb 2014; 32: 467.
PD-L1 expression in non-clear cell renal cell carcinoma.
ASCO Meeting Abstracts, Feb 2014; 32: 424.
PD-L1 limits the mucosal cd8+ t cell response to chlamydia trachomatis
J. Immunol., Feb 2014; 192: 1079 - 1090.
PD-L1 expression in triple negative breast cancer
Cancer Immunology, Jan 2014; 10.1158/2326-6066.CIR-13-0127.




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Antibodies:
Purified Polyclonal
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