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Human PD-1 (PDCD1) Recombinant, Biotinylated

PD-1

accession:  Q15116
   Size A     10ug   $ 340
   Size B      25ug  $590
   Size C      100ug  $ 1950
Domain  : 
Gene  :  PDCD1
Catalog no. :  RKQ15116

 

Source:

Optimized DNA sequence  encoding  extracellular domain of Human Programmed cell death protein 1 PD-1 (PRO21 - GLN167) including a C-terminal His tag was expressed in HEK293 cells.

Recombinant Human PD-1 was further labeled by Biotin for detection by Avidin conjugated secondary probes.

 
Molecular weight:

Recombinant human PD-1 (PDCD1) is a protein consisting of 150 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Applications:

ELISA,WB

Biotinylated recombinant PD-1 was designed to be used in single step detection in ELISA ,WB.

 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human PD-1 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Storage:
Recombinant  PD-1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

PD-1

The programmed cell death-1 (PD-1) is an inhibitory surface receptor expressed by T cells, B cells, natural killer T cells, monocytes, and dendritic cells (DC), but not by resting T cells. PD-1 binds two ligands, programmed cell death ligand 1 (PD-L1) and PD-L2 called B7-H1 and B7-DC, respectively . Tumors can use the PD-1 inhibitory pathway to silence the immune system

Related Publications:
differential impact of PD-1 and/or interleukin-10 blockade on hiv-1-specific cd4 t cell and antigen-presenting cell functions
J. Virol., Mar 2014; 88: 2508 - 2518.
PD-1 and tim-3 regulate the expansion of tumor antigen–specific cd8+ t cells induced by melanoma vaccines
Cancer Res., Feb 2014; 74: 1045 - 1055.
negative regulation by pd-l1 during drug-specific priming of il-22–secreting t cells and the influence of PD-1 on effector t cell function
J. Immunol., Feb 2014; 10.4049/jimmunol.1302720.
PD-1 blockage delays murine squamous cell carcinoma development
Carcinogenesis, Feb 2014; 35: 424 - 431.
highly pathological influenza a virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific cd8+ t cells
J. Virol., Feb 2014; 88: 1636 - 1651.




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Antibodies:
Purified Polyclonal
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