| Source: |
| Optimized DNA sequence encoding Human Neuregulin-4 EGF domain was expressed in Escherichia Coli. |
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| Molecular weight: |
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Recombinant human Neuregulin-4 EGF domain is a disulfide-linked monomeric protein consisting of 62 amino acid residue subunits, and migrates as an approximately 7 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
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| Purity: |
| >96%, as determined by SDS-PAGE and HPLC |
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| Biological Activity: |
| The ED(50) was determined by the phosphorylation of ErbB2 and ErbB4 receptors in CHO cells, and was found to be <3ng/ml, corresponding to a specific activity of 3x 105 Units/mg. |
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| Protein Sequence: |
10 20 30 40 50 60
MPTDHEEPCG PSHKSFCLNG GLCYVIPTIP SPFCRCVENY TGARCEEVFL PGSSIQTKSN
70 80 90 100 110 120
LFEAFVALAV LVTLIIGAFY FLCRKGHFQR ASSVQYDINL VETSSTSAHH SHEQH
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(*)Complete precursor sequence shown, expressed chain highlighted
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| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
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| Presentation: |
Recombinant Neuregulin-4 was lyophilized from a 0.2 μm filtered 20mM PB, pH 6.0.
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| Reconstitution: |
| A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
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| Storage: |
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles. |
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| Usage: |
| This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
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