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Human MCP-3 (CCL7) Recombinant , Biotinylated

MCP-3

accession:  P80075
   Size A     10ug   $ 340
   Size B      25ug  $590
   Size C      100ug  $ 1950
Domain  :  IL8
Gene  :  MCP3
Catalog no. :  RBP80098

(*)Complete precursor sequence shown, expressed chain highlighted
 

Source:
Optimized DNA sequence encoding Human MCP-3(CCL7) mature chain was expressed in Escherichia Coli. Recombinant Human CCL7 was further labeled by Biotin for detection by Avidin conjugated secondary probes.

 
Molecular weight:

Human MCP-3, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 9 kDa.

Recombinant MCP-3 is a  monomer protein consisting of  75amino acid residue subunits and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent chemo-attraction of  cells expressing CCR 2 and was found to be  2nM

 
Protein Sequence:
        10         20         30         40         50         60 
MKASAALLCL LLTAAAFSPQ GLAQPVGINT STTCCYRFIN KKIPKQRLES YRRTTSSHCP 

        70         80         90        100 
REAVIFKTKL DKEICADPTQ KWVQDFMKHL DKKTQTPKL 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
MCP-3 was lyophilized from a 0.2 μm filtered 20mM PB solution pH 7.5, 150mM NaCl.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

MCP-3



Related Publications:
reduced degradation of the chemokine MCP-3 by matrix metalloproteinase-2 exacerbates myocardial inflammation in experimental viral cardiomyopathy
Circulation, Nov 2011; 124: 2082 - 2093.
expression of ccr-3 ligands, rantes, MCP-3 and eotaxins is differentially regulated in human retinal pigment epithelial cells
Invest. Ophthalmol. Vis. Sci., Apr 2010; 51: 6163.
monocyte chemoattractant protein-1 (mcp-1), not MCP-3, is the primary chemokine required for monocyte recruitment in mouse peritonitis induced with thioglycollate or zymosan a
J. Immunol., Sep 2009; 183: 3463 - 3471.
additive roles for mcp-1 and MCP-3 in ccr2-mediated recruitment of inflammatory monocytes during listeria monocytogenes infection
J. Immunol., May 2008; 180: 6846 - 6853.
infection of hrpe cells with cmv induces the differential expression and secretion of mcp-1, MCP-3 and il-8
Invest. Ophthalmol. Vis. Sci., Dec 2002; 43: 4329.




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product_icon_8.jpg Human MCP-3 (CCL7) Recombinant
product_icon_8.jpg Human MCP-3 (CCL7) Recombinant , Biotinylated
 
 
 
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