| Source: |
| Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in Escherichia Coli. |
|
| Molecular weight: |
|
Native human IL-8, generated by the proteolytic removal of the signal peptide
and propeptide,the molecule has a calculated molecular mass of approximately 8 kDa.
Recombinant IL-8 is a disulfide-linked monomer protein consisting of 73 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
|
|
| Purity: |
| >95%, as determined by SDS-PAGE and HPLC |
|
| Biological Activity: |
| Determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 10.0-100.0 ng/ml. |
|
| Protein Sequence: |
10 20 30 40 50 60
MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH
70 80 90 100
CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS
|
(*)Complete precursor sequence shown, expressed chain highlighted
|
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
|
| Presentation: |
| Recombinant IL-8 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5 . |
|
| Reconstitution: |
| A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers |
|
| Storage: |
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles. |
|
| Usage: |
| This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
|
