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Human Interleukin-24 Recombinant |
IL-24 |
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| accession: |
Q13007 |
| Size A |
2ug |
$70 |
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| Size B |
10ug |
$160 |
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| Size C |
1mg |
$
3900 |
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| Domain : |
4 Helix Cytokine |
| Gene : |
IL24 |
| Catalog no. : |
RKQ13007 |
| Source: |
| Optimized DNA sequence encoding Human Interleukin-24 mature chain including a C-terminal His tag was expressed in HEK293 cells. |
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| Molecular weight: |
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Native human IL-24 has a calculated molecular mass of approximately 18.5 kDa.
Recombinant IL-24 is a disulfide-linked monomeric protein consisting of 165 amino acid residue subunits, and migrates as an approximately 35kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
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| Purity: |
| >95%, as determined by SDS-PAGE and HPLC |
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| Biological Activity: |
| Activity was determined by the ability to inhibit growth of human A549 cancer cells at concentrations ranging between 50-100 ng/ml. |
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| Protein Sequence: |
10 20 30 40 50 60
MNFQQRLQSL WTLARPFCPP LLATASQMQM VVLPCLGFTL LLWSQVSGAQ GQEFHFGPCQ
70 80 90 100 110 120
VKGVVPQKLW EAFWAVKDTM QAQDNITSAR LLQQEVLQNV SDAESCYLVH TLLEFYLKTV
130 140 150 160 170 180
FKNYHNRTVE VRTLKSFSTL ANNFVLIVSQ LQPSQENEMF SIRDSAHRRF LLFRRAFKQL
190 200
DVEAALTKAL GEVDILLTWM QKFYKL
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(*)Complete precursor sequence shown, expressed chain highlighted
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| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
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| Presentation: |
| Recombinant Interleukin-24 was lyophilized from 0.2μm filtered solution in PBS, pH 7.4 including 5% (w/v) trehalose. |
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| Reconstitution: |
| A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
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| Storage: |
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles. |
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| Usage: |
| This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
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IL-24 |
IL-24 (melanoma
differentiation-associated
gene-7 (mda-7)), a member of
the IL-10 cytokine family,
possesses the properties of
a classical cytokine as well
as tumor suppressor effects.
The exact role of IL-24 in
the immune system has not
been defined but studies
have indicated a role for
IL-24 in inflammatory
conditions such as
psoriasis. The tumor
suppressor effects of IL-24
include inhibition of
angiogenesis, sensitization
to chemotherapy, and p38
mitogen-activated protein
kinase (MAPK)-mediated
apoptosis. Current knowledge
on the regulation of IL-24
expression is sparse. The
melanoma differentiation-
associated gene-7 (mda-7)
was discovered through
subtraction hybridization of
cDNA libraries prepared from
melanoma cells.
As mda-7 shares considerable
structural and sequence
homology with IL-10 cytokine
family members it was
recognized as a member of
this family and renamed
interleukin-24 (IL-24).
IL-24 signals through a
heterodimeric receptor
complex consisting of the
IL-20Rβ subunit together
with either the IL-20Rα
subunit or the IL-22Rα
subunit. The IL-20Rα/IL-20Rβ
complex is shared with IL-19
and IL-20 whereas the IL-
22Rα/IL-20Rβ complex is
shared with only IL-20.
Binding of IL-24 as well as
IL-19 and IL-20 to the
receptor complexes results
in signal transducer and
activator of transcription 3
(STAT3) -activation.
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| Related Publications: |  | a serotype 5/3 adenovirus expressing mda-7/IL-24 infects renal carcinoma cells and promotes toxicity of agents that increase ros and ceramide levels
Mol. Pharmacol.,
Nov 2010;
10.1124/mol.110.069484.
|  | a single intramuscular injection of aav-8 vector expressing mda7/IL-24 efficiently suppresses tumor growth mediated by multiple anti-cancer mechanisms in lymphoma model mice.
Blood (ASH Annual Meeting Abstracts),
Nov 2010;
116:
1474.
|  | mechanism by which mcl-1 regulates cancer-specific apoptosis triggered by mda-7/IL-24, an il-10–related cytokine
Cancer Res.,
Jun 2010;
70:
5034 - 5045.
|  | IL-24 transgenic mice: in vivo evidence of overlapping functions for il-20, il-22, and IL-24 in the epidermis
J. Immunol.,
Feb 2010;
184:
1793 - 1798.
| | perk–dependent regulation of ceramide synthase 6 and thioredoxin play a key role in mda-7/IL-24–induced killing of primary human glioblastoma multiforme cells
Cancer Res.,
Feb 2010;
70:
1120 - 1129.
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