Optimized DNA sequence encoding extracellular domain of Human CD33 including a C-terminal His tag was expressed in HEK293 cells.
CD33 was further labled by Biotin for detection by avidin conjugated secondary probes.
Recombinant CD33 (Siglec-3) is a protein consisting of 250 amino acid residue subunits, due to glycosylation migrates as an approximately 40kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Biotinylated CD33 was designed to be used in single step detection in ELISA ,WB
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human CD33 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant CD33 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CD33 is a 67-kDa
that contains one V-set and
one C2-set Ig-like domain6
and is specifically
expressed on the myeloid
lineage. It is a biomarker
and therapeutic target for
acute myeloid leukemia
sgn-CD33a: a novel CD33-targeting antibody–drug conjugate using a pyrrolobenzodiazepine dimer is active in models of drug-resistant aml
1455 - 1463.
CD33 alzheimer's risk-altering polymorphism, CD33 expression, and exon 2 splicing
13320 - 13325.
cd14+CD33+ myeloid cell-ccl11-eosinophil signature in ulcerative colitis
J. Leukoc. Biol.,
blood CD33(+)hla-dr(–) myeloid-derived suppressor cells are increased with age and a history of cancer
J. Leukoc. Biol.,
633 - 637.
clinical significance of CD33 nonsynonymous single-nucleotide polymorphisms in pediatric patients with acute myeloid leukemia treated with gemtuzumab-ozogamicin–containing chemotherapy
Clin. Cancer Res.,
1620 - 1627.