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Human CD32b Recombinant ( FcgR2b), Biotinylated

CD32

accession:  P31994
   Size A     10ug   $ 340
   Size B      25ug  $590
   Size C      100ug  $ 1950
Domain  :  Ig-like C2
Gene  :  CD32B
Catalog no. :  RBP31994

Source:

Optimized DNA sequence  encoding Human extracellular domain of human CD32b including a C-terminal 6His tag was expressed in HEK293 cells.

CD32b was further labled by Biotin for detection by avidin conjugated secondary probes.


 
Molecular weight:

Recombinant CD32b is a monomer protein consisting of 183 amino acid residue subunits,  due to glycosylation migrates as an approximately 30 kDa protein on SDS-PAGE.


Applications:

ELISA,WB

Biotinylated CD32b  was designed to be used in single step detection in ELISA ,WB

 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant CD32b  is supplied as a lyophilized 0.2 μm filtered PBS solution,  pH7.2 .

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.

 
Storage:
Recombinant CD32b, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD32b

Human CD32B (FcγRIIB), the low-affinity inhibitory Fcγ receptor (FcγR), is highly homologous in its extracellular domain to CD32A (FcγRIIA), an activating FcγR.CD32B is coexpressed with activating Fc receptors on monocytes, macrophages, basophils and mast cells. CD32B is also expressed by B lymphocytes, the sole FcγR expressed by these cells, where it functions by countering B- cell receptor (BCR)-induced activation.

Related Publications:
CD32b expression reflects phenotypic and functional heterogeneity in multiple myeloma (mm)
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 842.
CD32b is highly expressed on clonal plasma cells from patients with systemic light-chain amyloidosis and provides a target for monoclonal antibody–based therapy
Blood, Apr 2008; 111: 3403 - 3406.
an fc-optimized monoclonal antibody against CD32b, the human inhibitory fc- receptor iib, in the immunotherapy of b-cell malignanacy.
Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 2529.
CD32b on clonal plasma cells in systemic light-chain amyloidosis (al) and multiple myeloma (mm): a target for immunotherapy in both disorders and a prognostic marker in mm.
Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 3489.




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Antibodies:
Purified Polyclonal
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