Optimized DNA sequence encoding Human extracellular domain of human CD16b including a C-terminal 6His tag was expressed in HEK293 cells.
CD16b was further labled by Biotin for detection by avidin conjugated secondary probes
Recombinant CD16b is a monomer protein consisting of 195 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Biotinylated CD16b was designed to be used in single step detection in ELISA ,WB
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD16b is supplied as a lyophilized 0.2 μm filtered PBS solution, pH7.4 containing 5% trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Recombinant CD16b, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20° to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Human CD16 is encoded by two
genes, FcγRIIIA and
FcγRIIIB. FcγRIIIa is a
transmembranous isoform of
the receptor expressed on
the surface of natural
killer (NK) cells,
macrophages, a subset of
monocytes and of T cells in
association with dimers of
the γ-chain of FcεRI and/or
ζ-chain of T-cell receptors.
FcγRIIIb is a
-anchored isoform restricted
to neutrophils. FcγRIII
plays a significant role in
the clearance of immune
cytotoxicity of K/NK
human basophils express the glycosylphosphatidylinositol-anchored low-affinity igg receptor fcriiib (CD16b)
2542 - 2550.